The LIS (Liquid Injection System) is a cutting-edge solution for automated, parallelized, and precise liquid feeding in shake flask cultures. Designed for simplicity and efficiency, the LIS enables researchers to implement custom feeding strategies while maintaining optimal culture conditions. Compatible with a wide range of liquids, including sugars, alcohols, acids, and bases, the system streamlines shake flask experiments and improves reproducibility.

The LIS system consists of three key components: the drive, the sterile cartridge, and optional LIS software for wireless monitoring and control. Predefined feeding profiles allow quick setup, while the software enables remote control, monitoring, and custom profile creation for time- or event-based feeding strategies. Integration with eve® software ensures feeding profiles can be managed alongside other bioprocess parameters and logged in a central database for analysis.

Key Features and Benefits

• Automated and parallelized feeding for multiple shake flasks

• Sterile single-use cartridge holds up to 25 mL of feeding liquid

• Cartridge fits all shake flasks, typically sealed with a metal cap

• Programmable miniature pump (drive) controls feed rate and timing

• Feeding profiles are configurable manually or wirelessly via LIS software

• Supports exponential and time/event-based feeding strategies

• Compatible with a wide range of liquids: sugars, alcohols, acids, and bases

• Remote monitoring and control via eve® software

• Feeding data logged and integrated with the central bioprocess database

• Enhances reproducibility, precision, and efficiency of shake flask experiments

Applications

• Automated feeding in shake flask cultures

• Microbial fermentation and growth optimization

• Mammalian, plant, or microbial cell culture experiments

• Process development and optimization

• Time- or event-based feeding studies